Device

Part:BBa_K1982006:Design

Designed by: Zexu Li   Group: iGEM16_NEU-China   (2016-09-12)


tCas9-Vp64(Prokaryotic)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 3667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3786
    Illegal SapI.rc site found at 1177
    Illegal SapI.rc site found at 1419


Design Notes

mutant Pst 1 :gctacctgcaG to gctacctgcaC

mutant Pst 1 :actatctgcaG to actatctgcaC

Source

tCas9 is from GE Share company. VP64 is synthetic construct.

References